Conditionally Immortalised Glomerular Endothelial Cell Line

2 flasks of live conditionally immortalised glomerular endothelial cells. The human glomerular endothelial cell line (GEnC) is considered to be a gold standard in glomerular filtration research. GEnCs are highly specialised cells which form the inner layer of glomerular capillaries as part of the glomerular microcirculation. They are an essential component in glomerular filtration. With damage of GEnCs implicated in diabetic nephropathy, haemolytic uraemic syndrome and preeclampsia, in vitro study of GEnCs is vital to gain understanding of such diseases. However, as a difficult primary cell to culture, study to date has been limited. The development of a conditionally immortalised GEnC line allows the in vitro differentiation of cells in a similar manner to in vivo, without the onset of early senescence which limits other GEnC lines. This novel, stable, easy to culture cell line has key characteristics of GEnCs, including expression of markers such as PECAM1, ICAM2, VEGFR2, vWF, and, uniquely for a cell line, upregulates fenestrations in response to VEGF.

Please be aware:

• The cells arrive in 2 x T25 culture flasks, about 2 million cell/flask.
• These are live cells, not frozen.
• You will need an incubator and media ready for the arrival of the cells.
• Live cells are sent by FedEx but not dry ice shipping.
• Orders are prepared in batches (rather than preparing the cells for each collaborator individually) which may result in a delay of up to 2 months from payment date in sending the cells.
• During checkout, we ask that you provide us with any known closure dates/holidays over the next two months that mean you would be unable to receive the cells.
• During checkout, you will be asked to confirm that you have checked if import permission is required by your shipping destination, and arranged if necessary to do so.